JACC: Basic to Translational Science
Cardiomyocyte SMAD4-Dependent TGF-β Signaling is Essential to Maintain Adult Heart Homeostasis
Prachi Umbarkar, Anand P. Singh, Manisha Gupte, Vipin K. Verma, Cristi L. Galindo, Yuanjun Guo, Qinkun Zhang, James W. McNamara, Thomas Force and Hind Lal
Figure 7
Figure 7
RNA-Seq Analysis Reveals Alteration in Ion Channel Genes in SMAD4 KO Mice
RNA sequencing (RNA-Seq) analysis was performed at 4 weeks after TAM treatment. (A) Hierarchical clustering of 161 genes detected as significantly differential (at least 1.5-fold, p value <0.05) between the CTL and SMAD4 KO groups. (B) Gene Ontology analysis. (C) Quantitative polymerase chain reaction analysis of ion-channel genes: kcna2 (***p = 0.0006), kcnd2 (*p = 0.0291), kcne1 (*p = 0.0111), clcn1 (**p = 0.0016), scn4b (***p = 0.0006), cacng6 (*p = 0.0130), and kcnv2 (**p = 0.0012). All p values are versus the CTL group. Abbreviations as in Figures 1 and 2.
Advertisement