Diabetes Exacerbates Myocardial Ischemia Reperfusion Injury by Down-Regulation of MicroRNA and Up-Regulation of O-GlcNAcylation
Dandan Wang, Xiaoyue Hu, Seung Hee Lee, Feng Chen, Kai Jiang, Zizhuo Tu, Zejian Liu, Jing Du, Li Wang, Chaoying Yin, Yu Liao, Hongcai Shang, Kathleen A. Martin, Raimund I. Herzog, Lawrence H. Young, Li Qian, John Hwa and Yaozu Xiang
(A) Quantitative polymerase chain reaction analysis of miR-24 expression in hearts of WT and transgenic mice [miR-24 tg] (normalized to U6). (B) Schematic protocol for myocardial I/R surgery. (C) Representative images of WT and transgenic mice myocardial tissue slices stained with Evans blue and triphenyl tetrazolium chloride. (D) Quantitation of infarct risk area. (E) Quantitation of infarct area expressed as percentage of the nonperfused risk area during coronary occlusion. Abbreviations as in Figure 1.