Diabetes Exacerbates Myocardial Ischemia Reperfusion Injury by Down-Regulation of MicroRNA and Up-Regulation of O-GlcNAcylation
Dandan Wang, Xiaoyue Hu, Seung Hee Lee, Feng Chen, Kai Jiang, Zizhuo Tu, Zejian Liu, Jing Du, Li Wang, Chaoying Yin, Yu Liao, Hongcai Shang, Kathleen A. Martin, Raimund I. Herzog, Lawrence H. Young, Li Qian, John Hwa and Yaozu Xiang
Overexpression of miR-24 Ameliorates Diabetic Ischemia Reperfusion Injury
(A) Schematic protocol of miR-24 delivery and I/R surgery. (B) Quantitative polymerase chain reaction analysis of miR-24 expression in hearts of db/db mice treated with mimic control (n = 6) or treated with miR-24 mimic (n = 6) for 2 weeks (normalized to U6). (C) Myocardial infarction size assessed following in vivo I/R (20 min LAD ligation and 3 h reperfusion) after treatment with miR-24 mimic (5 mg/kg) or scramble control intravenously, 2 weeks before LAD ligation. Representative images of myocardial tissue slices stained with Evans blue and triphenyl tetrazolium chloride. (D) Quantitation of infarct risk area. (E) Quantitation of infarct area expressed as percentage of the nonperfused risk area during coronary occlusion. (F) Enzyme-linked immunosorbent assay analysis of fasting plasma insulin levels in WT and db/db mice with without miR-24 mimic delivery (n = 8 to 10). (G) Fasting plasma glucose levels in db/db mice with without miR-24 mimic delivery (n = 10 to 12) (paired t test). Abbreviations as in Figure 1.