Ly6ChiCCR2+ Monocyte Depletion Suppresses Cardiac and Lymphoid T-Cell Expansion During Chronic Pressure-Overload HF
(A) Flow cytometry gating strategy for identification of cardiac T cells and CD4+ Th1 (IFN-γ+), Th2 (IL-4+), and T-regulatory (Foxp3+) subsets. (B) Representative gate plots of cardiac CD4+ T cells and corresponding tissue quantitation in mice treated with MC21 or IgG 4 weeks after TAC or sham operation. Statistical comparisons for CD4+ T cells performed after logarithmic data transformation as described in text. Quantitation of cardiac CD4+ T-cell subsets (C) and CD3+CD4−CD8+ cardiac T cells (D) in the same groups. (E) Top, representative gate plots of CD4+ and CD8+ T cells in mediastinal lymph nodes (MLN) from IgG- and MC21-treated TAC mice. Bottom, quantitation of CD3+, CD3+CD4+CD8−, and CD3+CD4−CD8+ T cells in the MLN of mice treated with MC21 or IgG 4 weeks after TAC or sham operation. *p < 0.05; n = 6 to 8 per group.