Pharmacological CCR2 Antagonism Prevents Cardiac Macrophage Infiltration in Early Pressure-Overload
(A) Experimental design for treatment with the CCR2 antagonist RS-504393 after transverse aortic constriction (TAC). (B) Quantitative group data for CCR2+ and CCR2− cardiac macrophages in mice treated with CCR2 antagonist or vehicle 1 week after TAC or sham operation. Statistical comparisons for CCR2+ macrophages were performed after logarithmic data transformation as described in the text. Group data for LV ICAM-1 and VCAM-1 gene expression (C) and Ly6Chi blood monocytes (D) in the same experimental groups. (E) Quantitative group data for total CD3+, CD3+CD4+, and CD3+CD8+ T-lymphocytes in mediastinal lymph nodes (MLNs) from the same animal groups. *p < 0.05, **p < 0.01, ***p < 0.001; n = 6 to 9 per group.