(A) Mouse LV gene expression of CCR2 chemokine ligands (left) and proinflammatory and anti-inflammatory cytokines (right) 1 week after transverse aortic constriction (TAC) or sham operation. (B) Identification of CD45+F4/80+MerTK+CD64+ cardiac macrophages using flow cytometry, and representative gate plots of CCR2− resident and CCR2+ infiltrating macrophages in TAC and sham mice. (C) Quantitation of CD45+ leukocytes, F4/80+MerTK+CD64+ macrophages, and CCR2+ and CCR2− macrophage subsets isolated from mouse hearts 1 week after TAC or sham operation. (D) Left, representative flow cytometry gate plots of Ly6Chi and Ly6Clow blood monocytes pre-gated on CD45+CD11b+Ly6G− cells from TAC and sham-operated mice (left) and quantitation of monocyte subsets (right). Ly6Chi monocytes uniformly express CCR2. *p < 0.05, **p < 0.01 versus sham; n = 6 per group.