IL-17A Promotes Inflammatory Cytokine Expression in Endothelial Cells
(A) IL-17 receptor A (Il17ra) and auxiliary subunits Il17rc and Il17re mRNA expression was determined by using quantitative polymerase chain reaction (qPCR). Il17rb messenger ribonucleic acid (mRNA) was below detection limit (n = 4, 2 exp.). (B to I) Endothelial cells were stimulated for 2 hours with 50 ng/ml IL-17A (B to E) or IL-17F (F to I). CCL2 (B and F), CXCL1 (C and G), IL-6 (D and H), and granulocyte-macrophage colony-stimulating factor (GM-CSF) (E and I) cytokine expression quantified by using qPCR (n = 8 from 4 independent experiments for each cytokine).