NDNF Rejuvenated Old hBM-MSCs and Improved Cardiac Function Through Activation of p-Akt
(A) The phosphorylated Akt (p-Akt) level in old NDNF-transduced hBM-MSCs (old+NDNF) was significantly higher than in empty vector–transduced cells (old). (B) Western blotting showed the expression of p-Akt protein in the border area of mouse heart tissue after implantation of control medium (medium), empty vector–transduced old hBM-MSCs (old), NDNF-transduced old hBM-MSCs (old+NDNF), or untransduced young hBM-MSCs (young) into the border region immediately following myocardial infarction (MI). The p-Akt level was significantly higher in the old+NDNF group than in the old group, n = 5/group. Abbreviations as in Figures 1 and 3.