In Vivo Engraftment of NDNF Overexpressing Old hBM-MSCs Increased Cell Survival and Decreased Apoptosis in the Heart After MI
(A) Representative heart tissue micrographs of immunofluorescent staining for human mitochondria (red) from mice that received implantation of empty vector–transduced old hBM-MSCs (old), NDNF-transduced old hBM-MSCs (old+NDNF), or untransduced young hBM-MSCs (young) into the border region immediately following myocardial infarction (MI). Nuclei are stained blue with DAPI. Quantification of surviving donor cells showed that NDNF overexpression improved cell survival. (B) Western blot analysis of the expression of NDNF protein in the border area of mouse hearts revealed significantly higher expression in the old+NDNF and young groups compared with the old and medium control groups. (C) Representative heart tissue micrographs of immunofluorescent staining for TUNEL (red) with nuclei stained blue with DAPI. Significantly fewer TUNEL+ cells were found in the ischemic area of old+NDNF hearts than in old hearts. (D) Western blot analysis of the expression of the proapoptotic protein Bax in the ischemic area of mouse hearts revealed significantly lower expression in the old+NDNF group than in the old group, n = 5/group. Abbreviations as in Figures 1 and 3.