In Vivo Engraftment of NDNF Overexpressing Old hBM-MSCs Decreased Scar Size and Increased Angiogenesis After MI
Representative heart sections 28 days after myocardial infarction (MI) (white arrows indicate scar area) in mice that received implantation of control medium (medium), empty vector–transduced human dermal fibroblasts (fibro), NDNF-transduced human dermal fibroblasts (fibro+NDNF), empty vector–transduced old hBM-MSCs (old), NDNF-transduced old hBM-MSCs (old+NDNF), or untransduced young hBM-MSCs (young) into the border region immediately following MI. (B) Representative heart slides stained with Masson’s trichrome and planimetry-based quantification revealed that the scar size area was largest in the medium and fibro groups 28 days after MI. The scar size was significantly larger in the fibro+NDNF and old groups 28 days after MI than in the young and old+NDNF groups. (C) Left ventricular (LV) internal end-systolic dimension (LVIDs) was significantly lower in the young and old+NDNF groups 28 days after MI and (D) LV internal end-diastolic dimension (LVIDd) was significantly lower in the young group. Representative micrographs of heart tissue showing (E) capillary (stained with isolectin, red) and (F) arteriole (stained with isolectin, red) densities. Densities were significantly higher in the old+NDNF group than in the old group, n = 5/group. SMA = smooth muscle actin. Abbreviations as in Figure 1.