Suppression of Arrhythmia by Enhancing Mitochondrial Ca2+ Uptake in Catecholaminergic Ventricular Tachycardia Models
Maria K. Schweitzer, Fabiola Wilting, Simon Sedej, Lisa Dreizehnter, Nathan J. Dupper, Qinghai Tian, Alessandra Moretti, Ilaria My, Ohyun Kwon, Silvia G. Priori, Karl-Ludwig Laugwitz, Ursula Storch, Peter Lipp, Andreas Breit, Michael Mederos y Schnitzler, Thomas Gudermann and Johann Schredelseker
Efsevin Does Not Affect Beta-Adrenergic Stimulation in RyR2R4496C/WT Cardiomyocytes
cAMP accumulation in freshly isolated RyR2R4496C/WT cardiomyocytes was measured in response to different stimuli. Efsevin had no effect on cellular cAMP levels (p > 0.999, Kruskal-Wallis test), whereas ISO induced a significant increase in cellular cAMP (p < 0.001). This increase could be blocked using the beta-adrenoreceptor blocker propranolol (prop) (p = 0.036), although efsevin had no effect on the ISO-induced increase in cellular cAMP (p = 0.999), indicating that efsevin neither activates nor blocks the beta-adrenergic system in cardiomyocytes. Abbreviations as in Figure 1.