Natriuretic Peptide Receptor-C Protects Against Angiotensin II-Mediated Sinoatrial Node Disease in Mice
Martin Mackasey, Emmanuel E. Egom, Hailey J. Jansen, Rui Hua, Motahareh Moghtadaei, Yingjie Liu, Jaspreet Kaur, Megan D. McRae, Oleg Bogachev, Sara A. Rafferty, Gibanananda Ray, Adam W. Kirkby and Robert A. Rose
Effects of Ang II on AP Morphology and Hyperpolarization-Activated Current (If) in Isolated SAN Myocytes From Wild-Type and NPR-C−/− Mice
(A) Representative spontaneous AP recordings in wild-type and NPR-C−/− SAN myocytes treated with saline or Ang II. (B, C) Summary data illustrating cycle length (B) and DD slope (C) in wild-type and NPR-C−/− mice treated with saline or Ang II. *p < 0.001 versus saline; p = 0.557 for AP cycle length and p = 0.517 for DD slope between genotypes within treatment group; n = 16 cells for wild type/saline, 11 cells for wild type/Ang II, 10 cells for NPR-C−/−/saline, and 13 cells for NPR-C−/−/Ang II for AP data. See Supplemental Table S2 for additional AP parameters. (D) Representative If recordings in SAN myocytes isolated from wild-type and NPR-C−/− mice treated with saline or Ang II. (E) (F) Summary If IV curves for wild-type (E) and NPR-C−/−(F) mice treated with saline or Ang II. *p < 0.001 versus saline at each membrane potential by 2-way repeated-measures analysis of variance with Tukey post-hoc test. (G, H) If activation curves for wild-type (G) mice and NPR-C−/−(H) mice treated with Ang II. See Supplemental Figure S4 for additional If kinetic analysis. n = 17 cells for wild type/saline, 13 cells for wild type/Ang II; 13 cells for NPR-C−/−/saline, and 16 cells for NPR-C−/−/Ang II. IV = current voltage relationship; pA = picoamperes; pF = picofarads; other abbreviations as in Figures 1 and 2.