Novel Adult-Onset Systolic Cardiomyopathy Due to MYH7 E848G Mutation in Patient-Derived Induced Pluripotent Stem Cells
Kai-Chun Yang, Astrid Breitbart, Willem J. De Lange, Peter Hofsteen, Akiko Futakuchi-Tsuchida, Joy Xu, Cody Schopf, Maria V. Razumova, Alex Jiao, Robert Boucek, Lil Pabon, Hans Reinecke, Deok-Ho Kim, J. Carter Ralphe, Michael Regnier and Charles E. Murry
Generation and Characterization of Patient-Specific EHTs
(A) Schematic (left) and photograph (right) of polydimethylsiloxane molds used to create engineered heart tissue (EHTs). (B) Bright-field image of EHTs in culture. Vertical bars are pins used to prevent EHTs from migrating out of the mold. (C) EHT immunofluorescence with α-actinin, f-actin (phalloidin), and nuclear staining. (D, E) Quantitative analysis of cytoskeletal alignment of EHTs conditioned with static stress. Vector of stress conditioning is parallel to EHT edge (horizontal). *p < 0.05 (Student’s t-test). Error bars indicate SEM.