Novel Mechanistic Roles for Ankyrin-G in Cardiac Remodeling and Heart Failure
Michael A. Makara, Jerry Curran, Ellen R. Lubbers, Nathaniel P. Murphy, Sean C. Little, Hassan Musa, Sakima A. Smith, Sathya D. Unudurthi, Murugesan V.S. Rajaram, Paul M.L. Janssen, Penelope A. Boyden, Elisa A. Bradley, Thomas J. Hund and Peter J. Mohler
AnkG Levels Are Altered During Pressure Overload–Induced Cardiac Remodeling and HF
(A and C) 190-kDa ankyrin-G (AnkG) protein expression levels are significantly increased in wild-type (WT) mouse hearts following 2 weeks transverse aortic constriction (TAC) (n = 3 for both groups; p < 0.05). (B and C) AnkG protein levels are significantly reduced in WT mouse hearts following 6 weeks TAC (n = 3 for both groups; p < 0.05). In C, densitometry was performed of full-length, mature, 190-kD AnkG (vs. lower molecular weight products). (D) AnkG immunostaining (3-dimensional composite of Z-stacks) in WT myocytes at baseline (BL) and 2 weeks post-TAC visualized using a ratiometric filter to compare intensity. Note the striking increase in AnkG expression at the intercalated disc of the 2-week TAC myocyte. Scale bars = 20 μm.