Novel Mechanistic Roles for Ankyrin-G in Cardiac Remodeling and Heart Failure
Michael A. Makara, Jerry Curran, Ellen R. Lubbers, Nathaniel P. Murphy, Sean C. Little, Hassan Musa, Sakima A. Smith, Sathya D. Unudurthi, Murugesan V.S. Rajaram, Paul M.L. Janssen, Penelope A. Boyden, Elisa A. Bradley, Thomas J. Hund and Peter J. Mohler
N-cadherin (green) and AnkG staining (red) in the left ventricle of (A) non-failing human heart, as well as hearts from individuals with (B) ischemic HF and (C) nonischemic HF. In A, arrows note strong localization of AnkG at the intercalated disc. In B and C, asterisks denote AnkG loss. Nuclei are visualized in blue (bar = 10 μm). Immunoblot analysis demonstrates a decrease in total AnkG expression in (D) ischemic and (E) nonischemic heart failure compared with nonfailing hearts (n = 3 NF; n = 5 IHF; n = 5 NIHF; p < 0.05). AnkG = ankyrin-G; HF = heart failure; IHF = ischemic heart failure; NF = nonfailing; NIHF = nonischemic heart failure.