CD137/NFATc1 Axis Was Directly Targeted by miR-145
(A) Relative mRNA expression levels of NFATc1 and CD137 in the miR-145 mimic- or inhibitor-treated group were determined by quantitative polymerase chain reaction. Values are mean ± SEM of 3 samples in each group; *p < 0.05. (B) Protein levels of CD137 and NFATc1 altered by TNF-α or 5-aza could be rescued by an miR-145 mimic or inhibitor. (C) Expression of eukaryotic vectors with or without CD137 or NFATc1 3ʹUTR regulated by the miR-145 mimic. (D) Luciferase assay of the CD137 and NFATc1 3ʹUTR or the corresponding mutation plasmid treated with the mimic. Values are mean ± SEM of 3 samples in each group; *p < 0.05. MUT-UTR = mutation plasmid; NFATc1 = nuclear factor of activated T cells 1; n.s. = nonsignificant; other abbreviations as in Figures 2, 4, 5, and 6.