IL-17A Blockade Normalizes Inflammation in Established Atherosclerotic Lesions in Renal Impairment
(A to E)LDLr–/– mice after RI or ctrl were kept on a high-fat diet for a total of 12 weeks after RI. After 6 weeks, they were treated with anti–IL-17A antibody or isotype control. (B and C) Atherosclerotic root lesion size was assessed by histology (B, examples and [C] statistical analysis of n = 7 to 8 per group from 3 to 4 independent experiments, 2-way ANOVA, significant effects of treatment group [p = 0.018] and distance [p < 0.0001], no significant interaction). (D) Aortic roots were stained for CD11b (green) and CD11c (red) with nuclear counterstain (DAPI, blue) (typical examples, 10× and 20× original magnification, bars indicate 500 μm). (E) Flow cytometry of aortic leukocytes was used to measure the number of CD11b+ myeloid cells, CD11b+CD11c+, and CD11b+CD11c+MHCIIHIGH cells (n = 5 to 7 per group from 3 independent experiments, Bonferroni after ANOVA). Abbreviations as in Figures 1, 2, and 3.