Endocardial TRPC-6 Channels Act as Atrial Mechanosensors and Load-Dependent Modulators of Endocardial/Myocardial Cross-Talk
Vesna Nikolova-Krstevski, Soeren Wagner, Ze Yan Yu, Charles D. Cox, Jasmina Cvetkovska, Adam P. Hill, Inken G. Huttner, Victoria Benson, Andreas A. Werdich, Calum MacRae, Michael P. Feneley, Oliver Friedrich, Boris Martinac and Diane Fatkin
(A) Immunofluorescence evaluation of transient receptor potential channel (TRPC-6) (green) in atrial tissue from mice subjected to thoracic aortic constriction (TAC) (bottom row) and in sham-operated animals (top row). In sham-operated mice, endocardial TRPC-6 is present as a thin green line, co-localizing with the endothelial-specific cell-membrane marker PECAM-1 (red). Fourteen days post-TAC, endocardial TRPC-6 was mostly located around and within the nuclei. Similar re-distribution of endocardial TRPC-6 was seen in human right atrial tissue samples (B) from patients with (bottom row) and without (top row) a history of atrial fibrillation (AF). Cell nuclei are outlined by 4′,6-diamidino-2-phenylindole (blue). Scale bar = 10 μm.